General Laboratory equipment

Fig: Autoclave

Autoclave: Autoclave is a sealed devise that allows the entrance of steam under pressure. The use of moist heat facilitates killing of all microorganisms including heat resistance endospores. The underlying principle is, with increase in steam pressure, the temperature also rises. The water boils at 100°C upon the vapor pressure of atmosphere. Temperature will increase if vapor pressure is increased. The autoclave is operated at 15lb/inch2 for 30 minutes. This temperature for a period of 30 minutes is sufficient to kill all the vegetative and spore cells of microorganism. Pressure temperature relationship is given as Read more