Oscillating magnetic field (OMF) is generated with electromagnet of alternating current. The intensity varies periodically according to frequency and type of wave in the magnet. OMF is applied in the form of constant amplitude or decaying amplitude sinusoidal wave. The magnetic field may be homogenous or heterogeneous. In homogenous field, field intensity (B) is uniform in the area enclosed by the magnetic field coil. In heterogeneous field, field intensity (B) is non – uniform with the intensities decreasing as distances from the center of the coil increases.
Inactivation of microorganism with OMF is achieved in milk, yogurt and orange juice. Only one pulse of OMF is adequate to reduce bacterial population between 102 and 103 cfu/g. The intensity of magnetic field required is 2 – 25 T in the frequency range of 5 – 500 Hz. Efficiency of OMF depend on magnetic field intensity, number of pulses, frequency and properties of food (thickness, resistivity, electrical conductivity).
Mechanism of microbial inactivation:
Weak OMF could loosen the bond between ions and proteins. Many proteins like enzymes, vital for cell metabolism contains ions. Effect of OMF on calcium ions bond in Ca+ binding proteins such as Calmodoulin, magnetic field causes plane of vibration to rotate that loosens the bond between Ca+ and Calmodoulin. OMF may couple energy into magnetically active parts of large critical molecule such as DNA. 5 – 50 T magnetic field intensity couple to 1 dipole energy per oscillation in DNA generate 10-2 to 10-3 ev energy. Several oscillations and collective assembly of enough local activation may result in breaking down of covalent bonds in DNA molecule and inhibition of growth of microorganism.
Factors affecting microbial inactivation:
1. Magnetic field: Magnetic field may stimulate inhibit growth and reproduction. Single pulse of 5 – 50 T and frequency of 5- 500 KHz reduce microorganism by 2 log cycle. Magnetic field can also effect membrane fluidity and other properties of cell. However, it is impossible to clearly state the microbial inactivation effect of magnetic field.
2. Electrical resistivity: To apply OMF, food need to have high electrical resistivity. The applied magnetic field intensity depends on the electrical resistivity and thickness of food. With larger magnetic field intensities, products with large resistivity and thickness is recommended.